• Senior Research Technician - Freeman Lab

    Location US-MA-Boston
    Job Posted Date 3 weeks ago(6/4/2018 5:29 PM)
    Job ID
    Hidden (24988)
    full time
  • Overview

    Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.


    We are seeking a highly motivated and dedicated full-time senior technician. The laboratory is focused on characterization of costimulatory molecules such as PD-L1 that regulate the immune response and their potential for cancer therapy by analyzing human tumors and using mouse models.


    Commonly performed tasks will include cell culture, lymphocyte proliferation assays and macrophage function assays, mouse injection and tumor monitoring, cell preparation from mouse and human tumors, FACS analysis, ELISA, RNA extraction, real time PCR, gene cloning, and immunohistochemistry staining. The research technician will be trained by lab personnel in multicolor flow cytometry, molecular biology and in vivo mouse work. About 20% effort will be spent on lab/administrative support activities such as ordering, organization, and shipping.

    Schedule: Full-time, Mon-Fri.


    The successful candidate will be enthusiastic, self-motivated, dedicated, possess excellent organizational skills, be attentive to detail, and capable of forming effective working relationships with members of the research team. A BA/BS or equivalent in Biology, or a related field is required.

    A minimum of 2 years of post-bachelor’s degree or master’s degree or if less time post-bachelors, a high degree of proficiency in flow cytometry, cellular immunological assays or mouse tumor models is required.

    Proficiency in flow cytometry and cellular immunological assays or alternatively, proficiency in mouse tumor models is necessary. Experience working with monoclonal antibodies, hybridoma cell cultures, serum-free CHO cell protein production, protein manipulations such as purification, fluorophore conjugations, and western blotting is highly desirable.

    We are looking for candidates who have at least two years of availability. Please submit 1) a cover letter briefly summarizing your research experience and career goals, CV, and contact information for three references.


    Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.


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