Dana-Farber Cancer Institute

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Research Technician - Freeman Lab

Research Technician - Freeman Lab

Job ID 
2017-8308
Location 
US-MA-Boston
Category 
Technician/Technologist
Type 
full time
Grade 
17
Job Posted Date 
9/13/2017

More information about this job

Overview

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

 

The Freeman Lab at the Dana-Farber Cancer Institute is looking for a highly motivated and dedicated full-time technician to support a research on immune evasion mechanisms to PD1 mAb therapy. The Freeman laboratory is focused on characterization of costimulatory molecules such as PD-L1 that regulate the immune response and their potential for cancer therapy using mouse models.

 

The successful candidate will be enthusiastic, self-motivated, dedicated, possess excellent organizational skills, be attentive to detail, and capable of forming effective working relationships with members of the research team.

 

We are looking for candidates who have at least two years of availability. Please submit 1) a cover letter briefly summarizing your research experience and career goals, CV, and contact information for three references.

Responsibilities

Responsibilities

  • The candidate will be responsible for the examination of animals, routinely monitoring tumor growth.
  • Isolate and prepare specimens for examination: blood, mouse tissue, cell lines and primary cells for sorting and flow cytometry analysis.
  • Maintenance of cell lines and set up, organize and perform CRISPR/CAS9 assay.
  • Perform various experiments including immunoblotting, immunohistochemistry, RNA-extraction, ELISA, real-time PCR, molecular biology (cloning, mutagenesis), protein purification.
  • Maintain detailed laboratory records, analyze data, and report experimental results.
  • Lab management, including ordering, maintaining laboratory equipment and preparing reagents and solutions.

Qualifications

Qualifications

  • A BA/BS or equivalent in Biology, or a related field is required.
  • The candidate should ideally have at least 2 years of post undergraduate research experience and must be a quick and willing learner. 
  • Must have excellent communication skills (written and verbal) and be able to work with a wide variety of faculty and staff. 
  • Proficiency in flow cytometry and molecular and cellular biology is necessary.
  • Experience with mouse tumor models, especially brain tumor, is highly desirable.
  • The successful candidate will be very organized, a team player, and able to work flexible hours in order to accommodate experimental needs.
  • Availability for 2 years is preferred

 

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.

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