Dana-Farber Cancer Institute

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Research Technician - Agudo Lab

Research Technician - Agudo Lab

Job ID 
full time
Job Posted Date 

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Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals. 


The Agudo Lab is focused on studying immune evasion mechanisms used by dormant disseminated cancer cells and cancer stem cells and discover means to allow the immune system to target them and prevent the growth of metastasis. We use mouse models of metastasis and a groundbreaking technology called Jedi T cells to dissect how or if T cells can recognize and eliminate pre-metastatic lesions.


The candidate will be required to perform basic laboratory duties, such as maintenance of laboratory instruments and equipment, preparation of solutions, organization of stocks and ordering. The candidate will also take care of mouse colonies, including breeding, tail clipping and PCR for genotyping and monitoring mice during the course of experiments (body weight and monitoring tumor growth). Moreover, the candidate will routinely perform key experiments, such as DNA cloning, lentiviral vector production, cell culture of cell lines and primary cells, western-blot, histology and tissue preparation for flow cytometry analysis.






  • Set up, adjust, calibrate, clean, maintain and troubleshoot equipment.
  • Clean, maintain and prepare supplies and work areas and may be responsible for ordering of supplies.  Collaborates with Purchasing and may be responsible for negotiating with vendors.
  • Conduct standardized biological, microbiological and biochemical analyses, such as DNA cloning, western-blot and ELISA.
  • Isolate and prepare specimens for examination: blood, mouse tissue, cell lines and primary cells for microscopy and flow cytometry analysis.
  • Maintenance of cell lines and set up, organize and perform viral vector production.
  • Monitors laboratory work to ensure compliance with Environmental Health and Safety information as well as the standard operation procedures specific to the laboratory.
  • Be deeply interested in immunology and/or cancer or stem cells (regenerative medicine). Expected to provide intellectual and interactive commitment to position by keeping up with appropriate literature and research publications.
  • Responsible for designing and executing advanced experiments and setting strategy.
  • Maintains detailed notebook of all work-related activities and may be responsible for the development, modification and improvement of standard operating procedures.
  • Will be responsible for the examination of animals, routinely monitoring to detect the presence of disease (such as cancer or autoimmunity).

Note:  The position will not start until October, 2017

This is a great opportunity for someone who wants to pursue a Ph.D.



  • Bachelor's Degree required with a major in biology or chemistry preferred. 
  • Must have excellent communication skills (written and verbal) and be able to work with a wide variety of faculty and staff.
  • Ability to analyze information and use logic to address work-related issues and problems.
  • Must have attention to detail and be thorough in completing work tasks.
  • Ability to work within a team environment.
  • Open to working with lab animals.
  • Previous lab experience is preferred
  • Availability for 2 years is preferred

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.

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