Senior Research Technician - Meyerson Lab

US-MA-Boston
4 months ago
Job ID
2017-7475
Category
Technician/Technologist
Type
full time
Grade
18

Overview

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

The positions in this job family will assist scientists in performing scientific research, development and/or clinical tasks of varying complexity. In general, they will assist in the set up, operation and maintenance of laboratory instruments and equipment, monitoring experiments, making observations and calculating and recording results.

Responsibilities

  • We seek an enthusiastic, responsible and highly capable laboratory technician, with a BA/BS in biology or a related field, and with two or more years of laboratory experience in molecular and cellular biology or biochemistry (ideally with some laboratory management experience), to join a cancer genomics research team that studies the molecular mechanisms of oncogenes in cancers, infection-associated cancers, and the interaction of the immune system with cancer. This individual will work with post-doctoral fellows using cutting-edge molecular biology and genomic techniques. The environment is highly collaborative, stimulating, and multi-disciplinary, and will provide a rich environment in which to advance skills in basic cancer biology, cancer genomics, and laboratory management and operations.

     

    The job will be split between laboratory management and research duties. Specific duties would include:

     

    1) Laboratory management and operations:

    • Maintaining stocks and inventories of laboratory supplies
    • Ordering laboratory supplies and reagents
    • Interfacing with vendors for purchases of equipment and reagents
    • Cataloging and organizing laboratory reagents
    • Managing equipment maintenance and repair
    • Other duties as appropriate

     

    2) Research:

    • Tissue culture of human cancer cells
    • DNA cloning and sequencing
    • Design and use of reagents for genome engineering with CRISPR/Cas9 technology
    • Extraction and analysis of DNA and RNA, including PCR, quantitative PCR, RT-PCR, and sequencing
    • Protein assays including immunoprecipitation and immunoblotting.
    • Flow cytometry
    • Enzymatic assays
    • Microbiological assays
    • Presenting findings at internal (DFCI) meetings
    Contributing to the development and execution of experiments and data analysis, in collaboration with the team.

 

 

Qualifications

  • Bachelor's degree required with a minimum of 2 years post degree research experience or a Master's degree.

  • We are seeking an exceptionally motivated scientist, who, in addition to appropriate technical and scientific knowledge, will have excellent organizational and interpersonal skills, a working knowledge of basic laboratory research software (e.g. Microsoft Word, Excel and Powerpoint), the ability to perform meticulous record keeping, be comfortable interacting with a diverse, energetic and collegial group of scientists, and enjoy managing as well as contributing to the laboratory’s research programs.
  • We are looking for someone with experience with basic molecular biology and biochemistry techniques, e.g. DNA & RNA isolation, cloning, plasmid preparation, Western blotting, tissue culture, gel electrophoresis, PCR, etc. In addition, knowledge of microbiological techniques e.g. bacterial isolation and culture, or advanced experimental techniques e.g. next-generation sequencing, FACS, ChIP-seq would be highly desirable and welcomed, as would be experience with mouse models of cancer.

This position will provide the ideal candidate with an excellent opportunity for intellectual growth, for mastering a variety of cutting-edge cellular and molecular biology techniques, and for contributing to advancing basic and translational cancer research, by collaborating with a productive, highly accomplished and cohesive research group.




Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.

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