Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.
The Marasco lab is looking for a highly motivated individual to perform hands-on bench research in the area of cancer immunotherapy. Our focus is on the development of therapeutic human antibodies and chimeric antigen receptor (CAR) T cells for the treatment of cancer. This individual will be an integral member of our research team and will be centrally involved in assisting different research projects related to modulating human T cells to more efficiently kill tumor cells. This position will include discovery research such as identifying new human antibodies through phage or yeast display technology that are active in restoring/enhancing anti-tumor activity of T cells. This position requires basic biochemical, molecular and cell biology skills including preparation and fluorescence activated cell sorting (FACS) of effector and regulatory T cell populations and accessory cells from peripheral blood and setting up in vitro assays to determine which antibodies restore anti-tumor activity. Other cellular assays include antibody dependent cellular cytotoxicity (ADCC), complement dependent cytotoxicity (CDC).
This is an exciting opportunity to be the part of a research team focused on the identification of clinically relevant human monoclonal antibodies and studies of their mechanism of action.
This is a regular, full time position. Nights and weekends may be required to meet research needs.
This individual will perform antibody panning experiments and screen positive clones by ELISA or flow cytometry, preparing samples for DNA sequencing and performing DNA sequence analysis, subcloning of antibody genes into mammalian/baculo expression vectors, production and purification of recombinant antibodies in mammalian cell or insect cell cultures, and their subsequent characterization including affinity analysis and testing biological activity for viral neutralization or in tumor inhibition. They will also provide assistance with construction and anti-tumor studies in humanized mice will also be required.
This individual is expected to make presentations at lab meetings to update the group on experimental progress or current literature and will be responsible for writing methods and materials, results, figure legends and constructing figures for grant proposals, progress reports and manuscripts. Intellectual input and co-authorship are encouraged. Other responsibilities include prepares and orders necessary supply stocks, independently operates lab equipment, and shares lab maintenance duties with other lab members.
BA/BS in biological sciences with background in molecular and cellular biology, immunology or microbiology. A preferred candidate would have prior laboratory research experience with strong background in both molecular and cell biology techniques including subcloning and production of recombinant proteins in E. coli, insect, and/or mammalian cells as well as subsequent biochemical and immunological analysis. Expertise in antibody discovery by phage and or yeast display are a plus. Proficient computer skills is also expected with competency in word, excel, etc. The applicant must be a self-starter with the ability to work well within a team environment. Once trained, applicant must be able to work on his/her own with minimal daily supervision. The individual is expected to be able to multi-task and be well organized, both require good time management skills; pay careful attention to experimental details, and have excellent communication skills.
Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.