• Scientist II - Marasco Lab in Cancer Immunology

    Location US-MA-Boston
    Job Posted Date 3 weeks ago(7/31/2019 7:15 AM)
    Job ID
    2019-16104
    Category
    Research- Laboratory
    Type
    full time
    Grade
    46
  • Overview

    Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

    Responsibilities

    Research Scientist Positions within the Department of Cancer Immunology and Virology are available to join our human antibody discovery and chimeric antigen receptor (CAR) T-cell cancer therapy programs. These research scientists will lead our efforts in developing CAR T cell factories and examining the combinatorial effect of CAR T with expression of the immune modulator antibodies on inhibiting tumor growth in vitro and in vivo. The focus of our efforts will be against ovarian cancer (OV) or triple negative breast cancer (TNBC).

    • Working with a team of dedicated research staff including computational, cellular, molecular and structural biologists and virologists, each research scientist will be responsible for designing and executing in vitro and in vivo experiments for their designated disease area.
    • S/he will generate cancer target specific CAR constructs, create CAR T-cells, carry out in vitro assays to test and optimize their biological activities, including generating appropriate tumor target cell lines, optimizing CAR T proliferation conditions, setting up in vitro target cell killing assays, characterizing CAR T cells’ responses to stimulation by target cells such as degranulation, cytokine production, cellular phosphorylation levels of intracellular signaling domains and activation induced cell death.
    • S/he is expected to set up appropriate tumor model(s) for their perspective project in humanized mice and perform in vivo experiments to evaluate therapeutic potential of specific CAR T-cell factory for a given cancer target.
    • In addition to monitoring tumor growth and metastases, s/he will assess the activity of CART cells (proliferation, exhaustion etc.), and analyze tumor microenvironment by transcriptional profiling using scRNAseq and other molecular means as well as multiparameter flow cytometry/immunohistochemistry.
    • The candidate must be a self-starter and highly motivated team player.
    • S/he is expected to work independently, well versed in the related literature, and contribute/lead intellectually to his/her research program.
    • The team member will also be responsible for providing regular written and oral presentations.
    • The successful candidate must have evidence of excellent English writing and verbal communications skills are required.

    Qualifications

    • Ph.D. degree with 3+ years of documented research experience in molecular biology and immunology, especially in antibody discovery and/or CAR T cell therapy.
    • Experiences in cancer animal model and relevant molecular, biochemical and immunological techniques are preferred.
    • We are particular interested in the candidates with prior research experience in the area of cellular immunology, gene-editing via CRISPER/CAS9, and/or specializing in mouse surgery and establishing human immune system reconstructed mouse models.
    • The individual is also expected to be able to parallel processing (good time management skills), well organized, and pay careful attention to experimental details.
    • While supervisory experience will be a plus, the candidate should expect to be fully involved in hands-on bench work.
    • Works in a busy academic research laboratory. Note that nights and weekends may be required to meet research needs.

    Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.

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