• Research Technician - Brown Lab

    Location US-MA-Boston
    Job Posted Date 1 month ago(5/6/2019 6:03 PM)
    Job ID
    2019-15125
    Category
    Hidden (24988)
    Type
    full time
    Grade
    17
  • Overview

    Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

     

    A full-time research technician position is available in the Department of Medical Oncology in the laboratory of Myles Brown. Our laboratory primarily studies the roles of steroid hormone receptors and epigenetic regulation in hormone-dependent cancers. For more information about our projects, please visit our website: https://mylesbrownlab.dana-farber.org/

     

    We are seeking a highly motivated research technician that will be an important member of our team and will work on multiple ongoing projects in the laboratory. The individual will primarily work with a postdoctoral fellow applying a variety of innovative laboratory techniques including CRISPR/Cas9 genetic screening, Chromatin Immunoprecipitation, next-generation sequencing library preparation, western blotting, in vivo mouse techniques, molecular biology cloning, and bioinformatic analysis. In addition to laboratory experiments, the position will also include some laboratory management and maintenance responsibilities. The candidate will be expected to independently analyze data, write up protocols and procedures, produce figures for manuscripts, give presentations at lab meeting, and attend departmental seminars and meetings. The ideal candidate would have at least two years of availability to dedicate to this role.

     

     

    Responsibilities

    Responsibilities

    • Tissue culture of human cancer cell lines
    • Protein, DNA, and RNA isolation
    • Western Blotting
    • Chromatin Immunoprecipitation
    • Next-generation sequencing library preparation
    • Molecular cloning and design of shRNA or CRISPR/Cas9 sgRNA constructs
    • PCR and real-time PCR
    • Cell viability, apoptosis, cell proliferation assays
    • Data analysis using bioinformatics tools
    • May be responsible for mouse handling, dosing, and colony maintenance
    • Ordering laboratory reagents and maintaining proper stock of all general lab supplies
    • Maintaining an up to date laboratory notebook with detailed records of experiments and procedures
    • Other resea

      Required Qualifications

      • Bachelor’s Degree in biological sciences
      • Previous laboratory research experience in basic cellular and molecular biology research is required
      • Excellent communication skills
      • Excellent analytical and logical skills
      • Ability to learn and grasp new concepts quickly
      • Ability to work collaboratively in a team environment
      • Must have attention to detail and be able to follow directions
      • Excellent organizational skills
      • Proficient in basic computer skills (i.e. Microsoft Word, Excel, Powerpoint, GraphPad Prism)
      • Familiarity with computational tools and experience working with command line is a plus

     

     

    Qualifications

     

    Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.

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