• Research Technician

    Location US-MA-Boston
    Job Posted Date 10 months ago(12/12/2018 10:32 AM)
    Job ID
    Hidden (24988)
    full time
  • Overview

    Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.


    We are looking for a self-motivated researcher to join a team of scientists and physician-scientists in the laboratory of Dr. Julie-Aurore Losman, who is appointed at Dana-Farber Cancer Institute and the Broad Institute of MIT and Harvard. In this critical role, the research associate will function as a member of a multi-disciplinary team of physician scientists and basic scientists working at the Dana-Farber Cancer Institute on a fast-paced, cutting-edge project focused on understanding how cancer cell metabolism shapes chromatin architecture, with the goal of finding novel therapeutic opportunities in hematologic malignancies and solid tumors.

    The individual hired should have a solid background in biology and a strong interest in molecular biology. This position would be ideal for those candidates interested in gaining significant experience in biomedical research with the goal of pursuing future graduate studies in medicine and/or research. In order to obtain sufficient training and experience, we are looking for candidates with at least 2 years of availability.

    This position will assist scientists in performing scientific research, development and/or clinical tasks of varying complexity. In general, this position will assist in the set up, operation and maintenance of laboratory instruments and equipment, monitoring experiments, making observations and calculating and recording results.

    Our laboratory studies the pathways that give rise to acute myeloid leukemia. We use biochemical assays, cell-based assays, and mouse models of human disease to identify novel tumor suppressors and oncogenes in leukemia, with a focus on understanding the mechanisms by which these genes promote cellular transformation. Our ultimate goal is to identify novel therapeutic targets in acute myeloid leukemia that will have clinical efficacy in patients.


    The candidate will be involved in a series of research activities, including building in vitro and in vivo human disease relevant models, experimental design, testing model systems in cellular and molecular assays, including in a high-throughput manner, use of next generation sequencing as well as molecular and cellular biology experiments. The candidate is expected to work largely independently on a daily basis after training and will be closely mentored by a post-doctoral fellow and by Dr. Losman. She/he will interact with and collaborate closely with other biologists, chemists and computational biologists at DFCI and the Broad Institute.

    Duties will include:

    • molecular biology techniques, including DNA and RNA isolation, PCR, and quantitative PCR
    • general husbandry and maintenance of mouse colonies
    • performing mouse bone marrow transplantations (tail vein injections, oral gavage, tissue dissection. 
    • flow cytometry
    • participation in general laboratory responsibilities, including ordering and organizing laboratory supplies and ensuring maintenance of equipment
    • Conduct standardized biological, microbiological or biochemical tests and laboratory analyses, including the collection of information and samples from organic substances, such as blood, drugs, and animals.
    • Isolate, identify and prepare specimens for examination
    • Analyze experimental data and interpret results to write reports and summaries of findings, including grant preparation and presentations, and look for opportunities to co-author publications. May be expected to participate in formal and information presentations of results to staff. 
    • Participate in grant application process and manuscript preparation as required
    • Maintains detailed notebook of all work-related activities.
    • Monitors laboratory work to ensure compliance with Environmental Health and Safety information as well as the standard operation procedures specific to each laboratory.
    • Expected to provide intellectual and interactive commitment to position by keeping up with appropriate literature and research publications.
    • molecular biology techniques, including DNA and RNA isolation, PCR, and quantitative PC
    • general husbandry and maintenance of mouse colonies
    • performing mouse bone marrow transplantations (tail vein injections, retro-orbital eye bleeds, oral gavage, mouse dissection.
    • isolation and purification of primary bone marrow cells from mice
    • flow cytometry
    • May be responsible for the development, modification and improvement of standard operating procedures.
    • Uses computers, computer-interfaced equipment, robotics or high-technology applications to perform work duties.
    • May be responsible for designing and executing advanced experiments and setting strategy. 
    • May be responsible for the examination of animals and specimens to detect the presence of disease or other problems.
    • May be responsible for monitoring laboratory budgets.




    • Bachelor's Degree required with coursework in related fields (such as Molecular Biology or Biochemistry) strongly preferred
    • Previous laboratory experience (including small animal work and some cellular and molecular laboratory techniques) is highly preferred, but all techniques will be taught upon joining the lab
    • Proficiency with computer software, including Power Point and Excel
    • Must be reliable, organized, and able to work independently as well as with other members of the team and the research center
    • Excellent communication and interpersonal skills
    • Knowledge of information and techniques related to either biology, chemistry, or medicine is preferred.
    • Must have excellent communication skills (written and verbal) and be able to work with a wide variety of faculty and staff.
    • Ability to analyze information and use logic to address work-related issues and problems.
    • Must have attention to detail and be thorough in completing work tasks.
    • Ability to work within a team environment.

    Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.



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