• Sr. Research Technician - Murakami Lab

    Location US-MA-Boston
    Job Posted Date 2 weeks ago(2 weeks ago)
    Job ID
    2018-12484
    Category
    Hidden (24988)
    Type
    full time
    Grade
    18
  • Overview

    Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

     

    We seek an experienced candidate for a full-time position integrating technician and operation management roles for a newly opened laboratory at the Dana-Farber Cancer Institute. The Murakami laboratory studies minimal residual disease in B-cell leukemia and lymphoma and aims to translate biologic insights into individualized therapies to improve clinical outcomes in patients with these diseases. We do this by utilizing our repository of preclinical models including patient-derived xenograft (PDX) mouse models, access to conventional and experimental therapeutic agents, expertise in lymphoid genomics and bioinformatics, novel single-cell biophysical and molecular profiling platforms, and involvement in early phase clinical trials. The candidate hired will work closely with Dr. Murakami to set up the laboratory, conduct experiments, and assist in ongoing laboratory management. Candidates are not expected to have personal experience in all the listed conceptual and methodological domains, but competitive candidates will be those who are eager to learn, flexible, willing to accommodate patient sample availability, highly organized, and possess excellent communication and record-keeping abilities.

    Responsibilities

    The senior technician will be responsible for conducting experiments to advance the research progress in the lab. As part of this work, they will be responsible for the following:

     

    Technical:

    • Preparation and maintenance of basic dry and wet chemicals and other common laboratory reagents
    • Extraction of nucleic acids and proteins from biologic specimens
    • Conventional PCR and quantitative RT-PCR
    • Immunoblotting
    • Maintenance of cell lines and primary cells in tissue culture
    • In vitro therapeutic studies in cell lines and primary samples, including in vitro proliferation and metabolic luminescence assays
    • Fluorescence- and magnetic-activated cell sorting (FACS and MACS, respectively)
    • Processing tissue samples from preclinical animal models and patients, including xenotransplantation, cryopreservation, cell fractionation using FACS or MACS, and extraction of nucleic acids and/or proteins
    • Generation of high-throughput sequencing libraries from DNA and RNA specimens
    • Mouse colony management, including breeding, irradiation, xenotransplantation, bleeding, dosing, and humane euthanasia
    • Maintenance of detailed records of laboratory work and willingness to communicate results over shared electronic communications platforms
    • Sharing responsibilities for operation and maintenance of common laboratory equipment

    Operations Management:

    • Ordering equipment for the laboratory based on discussions with Dr. Murakami
    • Ordering reagents for the laboratory based on recognized need and requests from other lab members
    • Assigning responsibility for basic lab jobs and maintenance of lab equipment to other lab members
    • Regular meetings with Dr. Murakami and the grants management office to review laboratory operations  

    The candidate should be able to perform basic laboratory work independently. Study design and the execution of more technically ambitious projects will be performed with Dr. Murakami and/or other trainees. Laboratory operation management will initially require close coordination with Dr. Murakami but is expected to offer greater latitude over time. The technician should have flexibility to work on some evenings and weekends as needed to meet project goals. We seek candidates who have at least 2 years of availability.

    Qualifications

    • Bachelor’s or Master’s degree in Biology, Biochemistry, Chemistry, or a related field is required.
    • At least 2 years of experience in a molecular biology lab and familiarity with some of the techniques listed above are required.
    • Excellent communication skills including timely responses to messages during business hours are required.
    • Ability to adjust work schedule based on clinical status of animals and availability of primary patient samples, sometimes on short notice, is required; longer hours on certain days will be counterbalanced on others.
    • Proficiency handling mice is strongly desired though not essential if candidate is willing to learn.
    • Ability to rotate with other lab members in caring for animal colony on weekends is required.

    Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.

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