• Research Technician

    Location US-MA-Boston
    Job Posted Date 1 week ago(6/14/2018 9:46 AM)
    Job ID
    Hidden (24988)
    full time
  • Overview

    Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.

    The Gimelbrant Laboratory is looking for a research technician to work in studying gene regulation in connection with cancer.  The lab currently uses and develops a variety of approaches, including allele-specific arrays and whole-transcriptome next-gen sequencing, in order to build a comprehensive map of allele-specific expression and the correlated chromatin states. This effort is under way in both human and mouse cells to gain an insight into the evolutionary context of allelic silencing. Of particular interest to us is the contribution of allelic inactivation to progression of malignancy; an epigenetic silencing of one allele is a functional equivalent of loss of heterozygosity, even as the genome is still apparently intact.  


    Another line of research is identification of the molecular mechanisms underlying the choice of active and inactive alleles. We are using stem cells to characterize the developmental plasticity of allele-specific expression. We are also developing mouse knock-in models for high-throughput loss of function screening of pathways that are involved in establishment and maintenance of epigenetically controlled allele-specific expression.


    • Primary tasks include
      • Maintain in vitro 3D tissue cultures (organoids).
      • Assist in experiments using deep sequencing based methods.
    • General lab maintenance tasks.
    • Order or generate laboratory reagents.

    This is a Full-time - 40 hours per week position with variable hours.  Some nights and weekends may be required to complete experiments and meet deadlines.


    • Bachelor's Degree in Biology or closely related field required.
    • Experience with mammalian cell culture is a strong preference.
    • Experience with molecular biology techniques, including with next generation sequencing approaches, is a plus.
    • Familiarity with data processing using command-line tools also a plus.
    • Ability to learn quickly, be well-organized and punctual
    • Must have excellent communication skills (written and verbal) and be able to work with a wide variety of faculty and staff.

    Ability to work within a team environment.
    Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.



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